Method and Device for Pathogen or Antibody Detection Using Unmodified Signaling Substances With or Without Unlabeled Detection Reagents

Technology #11472

Key Points

  • Quick and inexpensive methods of detecting pathogens or antibodies in biological samples such as blood, cerebrospinal fluid (CSF), tissue homogenates, cell suspension, urine, saliva
  • Does not require labeling or conjugating the detection reagent with the signaling molecule or substance to generate detectable signals
  • Has been used to screen for cytomegalovirus infection in urine samples

Abstract

The University of Central Florida invention relates to methods for detecting a target pathogen or antibody in biological samples. The disclosed methods include three embodiments and do not require labeling or conjugating of the detection reagent with the signaling molecule or substance to generate detectable signals. The first embodiment relates to using an unlabeled antibody reagent that is specific to the target pathogen. The second embodiment relates to using the pathogen as the detection reagent and the antibody as the target analyte. The third embodiment relates to using an unmodified signaling substance to detect the presence of inclusion bodies. The technology has been used to screen for cytomegalovirus infection in urine samples.