Method and Tools to Study Neuromuscular Junction

Technology #31152

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Flow diagram of a preferred method of the invention
James Hickman, Ph.D.
Hedvika Davis
Neelima Bhargava
Mainak Das, Ph.D.
Managed By
Brion Berman
Assistant Director 407.882.0342
Patent Protection

Method of myelinating isolated motoneurons

US Patent 8,828,721 B1

In Vitro Production Of Oligodendrocytes From Human Umbilical Cord Stem Cells

US Patent Pending 2012/0128639 A1

Method of co-culturing mammalian muscle cells and motoneurons

US Patent 9,650,606

Nervous system diseases such as amyotrophic lateral sclerosis, multiple sclerosis, and spinal cord injuries are debilitating conditions which currently have no effective treatments. Model systems which can effectively represent both neurons and muscles as well as the interaction between them are needed so that diseases can be studied and drug candidates can be accurately evaluated.

Technical Details

This invention is a functional in vitro model system for the study of the neuromuscular junction. Firstly, this includes a system for the culture of human motoneurons and muscle to form the neuromuscular junction. Also included are methods for co-culturing isolated motonuerons with Schwann cells in a chemically defined serum-free medium which supports myelination of the motonuerons, and culturing functional adult mammalian spinal cord neurons. Lastly, included are methods for the ability to produce oligodendrocytes from a human umbilical cord using a defined medium and a 3-D environment which allows for complete maturation of the cells.

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  • Serum free
  • Easy to manipulate
  • Compatible with BioMEM
  • Nodes of Ranvier formation


  • Study of: Spinal injuries, ALS, MS, demylinating diseases, and other motoneuron diseases

Additional Technology Numbers: 31821, 32132, 32604, 33024

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"In vitro Method of Myelinating Isolated Motoneurons" Technology Number: 31869