Preserve Nucleic Acids in Water Samples Without Refrigeration

Technology #2021-100

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Researchers
Sean Moore, Ph.D.
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US Patent Pending

Key Points

  • Method for rapidly preserving microorganisms and their nucleic acids on-site using simple collection vessels
  • No need for refrigeration or freezing
  • Can be used with larger volumes of fluids compared to liquid nucleic acid preservatives

Abstract

The University of Central Florida invention is a method for preserving nucleic acids in biological materials. It includes a dry nucleic acid preservative reagent that can be used on-site without refrigeration or freezing. The reagent is shelf-stable and does not interfere with the subsequent nucleic acid extraction. Additionally, due to the dry formulation, it can be used with larger volumes of fluids compared to liquid nucleic acid preservatives. This technology has been tested with seawater samples, and the recovered bacterial DNA was sufficient for amplification by polymerase chain reaction.

Benefit

  • Stable, non-hazardous, powder form
  • Simple to use, sample water is added to the dry formulation on-site
  • Allows large volumes of a water sample to be collected for nucleic acid analysis
  • Does not interfere with the subsequent extraction or analysis of nucleic acids

Market Application

  • Microbiological testing and analysis